transcription kit super script iii reverse transcriptase Search Results


ag pathid one step pcr kit  (Thermo Fisher)
86
Thermo Fisher ag pathid one step pcr kit
Ag Pathid One Step Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ag pathid one step pcr kit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
ag pathid one step pcr kit - by Bioz Stars, 2026-02
86/100 stars
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taqman microrna reverse transcription kit  (Thermo Fisher)
86
Thermo Fisher taqman microrna reverse transcription kit
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taqman microrna reverse transcription kit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
taqman microrna reverse transcription kit - by Bioz Stars, 2026-02
86/100 stars
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megascript high yield transcription kit  (Thermo Fisher)
86
Thermo Fisher megascript high yield transcription kit
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Megascript High Yield Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megascript high yield transcription kit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
megascript high yield transcription kit - by Bioz Stars, 2026-02
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high-capacity reverse transcription kit with omniscript rt universal primers (random decamers)  (Qiagen)
90
Qiagen high-capacity reverse transcription kit with omniscript rt universal primers (random decamers)
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
High Capacity Reverse Transcription Kit With Omniscript Rt Universal Primers (Random Decamers), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-capacity reverse transcription kit with omniscript rt universal primers (random decamers)/product/Qiagen
Average 90 stars, based on 1 article reviews
high-capacity reverse transcription kit with omniscript rt universal primers (random decamers) - by Bioz Stars, 2026-02
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mmulv reverse transcriptase and random hexamer primer (miscript ii rt kit)  (Qiagen)
90
Qiagen mmulv reverse transcriptase and random hexamer primer (miscript ii rt kit)
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Mmulv Reverse Transcriptase And Random Hexamer Primer (Miscript Ii Rt Kit), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmulv reverse transcriptase and random hexamer primer (miscript ii rt kit)/product/Qiagen
Average 90 stars, based on 1 article reviews
mmulv reverse transcriptase and random hexamer primer (miscript ii rt kit) - by Bioz Stars, 2026-02
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commercial kit quiagen onestep rt-pcr  (Qiagen)
90
Qiagen commercial kit quiagen onestep rt-pcr
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Commercial Kit Quiagen Onestep Rt Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/commercial kit quiagen onestep rt-pcr/product/Qiagen
Average 90 stars, based on 1 article reviews
commercial kit quiagen onestep rt-pcr - by Bioz Stars, 2026-02
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stratascript tm reverse transcriptase kit  (Qiagen)
90
Qiagen stratascript tm reverse transcriptase kit
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Stratascript Tm Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stratascript tm reverse transcriptase kit/product/Qiagen
Average 90 stars, based on 1 article reviews
stratascript tm reverse transcriptase kit - by Bioz Stars, 2026-02
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effectene transcription kit  (Qiagen)
90
Qiagen effectene transcription kit
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Effectene Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/effectene transcription kit/product/Qiagen
Average 90 stars, based on 1 article reviews
effectene transcription kit - by Bioz Stars, 2026-02
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reverse transcription kit 9619580410  (ABclonal Biotechnology)
90
ABclonal Biotechnology reverse transcription kit 9619580410
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Reverse Transcription Kit 9619580410, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reverse transcription kit 9619580410/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
reverse transcription kit 9619580410 - by Bioz Stars, 2026-02
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impro-iitm reverse transcription systerm  (Promega)
90
Promega impro-iitm reverse transcription systerm
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Impro Iitm Reverse Transcription Systerm, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/impro-iitm reverse transcription systerm/product/Promega
Average 90 stars, based on 1 article reviews
impro-iitm reverse transcription systerm - by Bioz Stars, 2026-02
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rt-pcr mix one-step access quick  (Promega)
90
Promega rt-pcr mix one-step access quick
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Rt Pcr Mix One Step Access Quick, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse reverse transcription kit  (Promega)
90
Promega mouse reverse transcription kit
Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any <t>microRNA.</t> NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)
Mouse Reverse Transcription Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse reverse transcription kit/product/Promega
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mouse reverse transcription kit - by Bioz Stars, 2026-02
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Image Search Results


Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any microRNA. NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)

Journal: Journal of Biomedical Science

Article Title: MicroRNA-1 induces apoptosis by targeting prothymosin alpha in nasopharyngeal carcinoma cells

doi: 10.1186/1423-0127-18-80

Figure Lengend Snippet: Luciferase assay demonstrates that miR-1 directly targets PTMA 3' UTR . (A) Schematic representation of the miR-1 targeting sequences that complement with the 3' UTR of PTMA mRNA. The PTMA 3'UTR (+779~801 and +973 ~ +997 nt, miR-1 predictive binding site) were cloned into pmirGLO luciferase vector to generate pmir-PTMA#1-wt and pmir-PTMA#2-wt. The miR-1 seed region complementary sequences were mutated and named as pmir-PTMA#1-mut and pmir-PTMA#2-mut. (B) NPC-TW01 cells were transfected with miR-1 or without any microRNA. NPC-TW01 cells were then transfected with pmirGLO empty vector, pmir-PTMA#1-wt, pmir-PTMA#2-wt, pmir-PTMA#1-mut or pmir-PTMA#2-mut. Luciferase assays were performed after 36 hours of transfection. The results indicate that miR-1 directly binds to the PTMA mRNA 3'UTR and impairs PTMA expression in NPC-TW01 cells. (*Comparison between two groups as indicated, p < 0.05. Error bars correspond to mean ± SD)

Article Snippet: For endogenous miR-1 quantification, microRNA was reverse-transcribed using a TaqMan MicroRNA reverse transcription kit (Applied Biosystems, Cat#4366597) and primers using the TaqMan endogenous controls RNU6B (Applied Biosystems, Cat#4373381) and TaqMan MicroRNA assays hsa-miR-1 (Applied Biosystems, Cat#4373161).

Techniques: Luciferase, Binding Assay, Clone Assay, Plasmid Preparation, Transfection, Expressing

PTMA siRNA and miR-1 accelerate the apoptotic process in cells were treated with actinomycin D . From top to bottom: untransfected NPC-TW01 cells (blank), transfection reagent only (mock), transfection reagent and negative control siRNA (Neg-si), transfection reagent and PTMA siRNA (PTMA-si), transfection reagent and miR-negative control (miR-neg) and transfection reagent and miR-1 (miR-1). Actinomycin D apoptotic inducer was added to culture media to a final concentration of 5 μM after 48 hours of transfection. Cells were subsequently observed by time-lapse microscopy (A). The time above each photograph represents the specific time that cells were transfected with microRNA, siRNA or control. (B) Cell viability was quantitated by MTT assay. The experimental conditions of the MTT assay were identical to those during time-lapse microscopy observation. Transfection of PTMA siRNA or miR-1 accelerated the apoptotic process after the NPC-TW01 cells were treated with actinomycin D apoptotic inducer.

Journal: Journal of Biomedical Science

Article Title: MicroRNA-1 induces apoptosis by targeting prothymosin alpha in nasopharyngeal carcinoma cells

doi: 10.1186/1423-0127-18-80

Figure Lengend Snippet: PTMA siRNA and miR-1 accelerate the apoptotic process in cells were treated with actinomycin D . From top to bottom: untransfected NPC-TW01 cells (blank), transfection reagent only (mock), transfection reagent and negative control siRNA (Neg-si), transfection reagent and PTMA siRNA (PTMA-si), transfection reagent and miR-negative control (miR-neg) and transfection reagent and miR-1 (miR-1). Actinomycin D apoptotic inducer was added to culture media to a final concentration of 5 μM after 48 hours of transfection. Cells were subsequently observed by time-lapse microscopy (A). The time above each photograph represents the specific time that cells were transfected with microRNA, siRNA or control. (B) Cell viability was quantitated by MTT assay. The experimental conditions of the MTT assay were identical to those during time-lapse microscopy observation. Transfection of PTMA siRNA or miR-1 accelerated the apoptotic process after the NPC-TW01 cells were treated with actinomycin D apoptotic inducer.

Article Snippet: For endogenous miR-1 quantification, microRNA was reverse-transcribed using a TaqMan MicroRNA reverse transcription kit (Applied Biosystems, Cat#4366597) and primers using the TaqMan endogenous controls RNU6B (Applied Biosystems, Cat#4373381) and TaqMan MicroRNA assays hsa-miR-1 (Applied Biosystems, Cat#4373161).

Techniques: Transfection, Negative Control, Concentration Assay, Time-lapse Microscopy, MTT Assay